Determination of the Structural Basis of Antibody Diversity Using NMR (Nuclear Magnetic Resonance)

摘要

The combination of Nuclear Magnetic Resonance (NMR) spectroscopy, recombinant DNA techniques and theoretical semiempirical model building appears to be a powerful approach to the protein engineering of antibody combining sites, for the ultimate production of antibodies having desired specificities and functions. In the past year we have sequenced the cDNA's of 12 monoclonal anti spin label antibodies, have constructed a working model for one of these antibodies (M. Levitt), and have carried out a number of NMR experiments that have tested this model and have provided kinetic data on hapten antibody binding. In the next year crucial tests of this model, our NMR assignments and this general approach to protein engineering will be made by transfection/expression experimentation involving specific mutations of the antibody genes for the antibodies ANO1-AN12. Keywords: Recombinant DNA, Monoclonal antibodies.