Protein Purification and Its Application to Crystallization

摘要

Structural studies on proteins depend on an investigator 's ability to isolate and purify the protein. In many cases, protein isolation is a trivial matter, but purification to homogeneity is a lengthy process. Traditionally, proteins have been purified by a combination of precipitation, open-column chromatography, including size-exclusion and ion-exchange chromatography, ultracentrifugation, and electrophoresis. Recently, high-performance liquid chromatography(HPLC)has become increasingly important in the purification and analysis of proteins. The purpose of this study was to apply modern HPLC techniques to the protein purification problems encountered in crystallographic studies. (aw)