Gene Regulation of Methanogenesis from Acetate in the Acetotrophic Methane Producing Archaebacteria

摘要

The research objectives of the project were to develop genetic and molecular techniques that will permit the detection, isolation, and cloning of genes that are regulated during acetate catabolism in the Methanosarcina spp. These studies should provide a basis for examining the regulation of acetate utilization by the methanogenic Methanosarcina. We have concentrated on three areas in the two year period of the contract. They are: 1) refining plating techniques and selecting for auxotrophic mutants; 2) characterizing plasmid(s) from Methanosarcina spp.; 3) creating a gene library from Methanosarcina spp.; 4) creating oligonucleotide and antibody probes and probing the chromosomal library for crossover dehydrogenase genes.