Nucleotide Sequence of the Protective Antigen Gene of Bacillus Anthracis

摘要

The DNA sequence of the protective antigen gene from Bacillus anthracis and the 5' and 3' flanking sequences were determined. Protective antigen is one of three proteins comprising anthrax toxin. The open reading frame is 2319 base pairs (bp) long, of which 2205 bp encode the 735 amino acids of the secreted protein. This region is preceded by 29 codons, which appear to encode a signal peptide having characteristics in common with those of other secreted proteins. A consensus TATAAT sequence was located at the putative -10 promoter site.