Estimating the Soluble Enzyme Activity in Brain Tissue. (Reannouncement with New Availability Information).

摘要

A quantitative estimate of the enzyme content of tissues from various organs is of biochemical interest and of potential clinical use. We have found that the amounts of enzyme measured in the brain are highly dependent upon the tissue processing techniques employed and that latent enzyme activity must be accounted for when estimating total enzyme content. Fresh rat cerebral tissue was subjected to simple homogenization, to freezing and thawing followed by homogenization, and to homogenization in 1% detergent solutions. The tissue enzyme concentrations, reported as U/g wet tissue weight, are always greatest in the detergent treated homogenates, next highest in the frozen-thawed homogenates, and least in the simple homogenates. Enzymes which are primarily cytosolic exhibit less marked increases in enzyme concentration with more efficient enzyme solubilization treatment than do enzymes associated wholly or in part with subcellular organelles such as lysosomes or mitochondria.