Metabolism, Mass Spectral Analysis and Mode of Action of Trichothecene Mycotoxins

摘要

The theoretical aspects of hybrid, tandem mass spectrometry is presented in the introduction with its application to multiple reaction monitoring. In summary, the desired parent mass fragment is transmitted through the sectoring portion of the instrument. When it reaches the collision chamber, it is fragmented into daughter ions in the environment of argon gas and voltage. The daughter ion fragments are unique for the compound in question; they are detected by the second quadrupole. Using the principles of tandem mass spectrometry described above, a method of analysis for T-2 toxin (trifluoroacetate derivative) in blood was developed. The parent fragments used was m/z+ 478 and the daughters that were monitored were m/z+ 180, 138 and 121. Deuterated internal standards were used for quantitative purposes. The TFA derivative of T-2 toxin was detected in whole blood at concentrations of 1, 10 and 20 parts per billion. This same method was used for quantitation of blood samples amended with T-2 toxin at concentrations of 1, 3 and 5 parts per billion. A linear plot of the quantitation is shown. Similar procedures were used for the detection of HT-2 toxin (trifluoroacetate derivative) in whole blood. Wortmannin is a natural toxic product produced by Fusarium oxysporum and F. sambucinum. The pathology of these toxins is presented. Pathological lesions in the heart, stomach, thymus and bladder were described. Keywords: Phytotoxins, Mycotoxins, Trichothecenes, Metabolism. (aw)