Immunological Techniques for Detection of Fungal and Dinoflagellate Toxins

摘要

Progress in the first year of the present contract is summarized as follows: 1) Antibody against group A trichothecenes was produced after immunization of rabbits with an immunogen prepared by conjugation of T-2 toxin to bovine serum albumin (BSA) at the C-8 position. 2) A monoclonal antibody against T-2 toxin was produced by fusion of P3-NS1/1-Ag4-1 myeloma cells with spleen cells of a BALB/c mouse which had been immunized with 3-Ac-NEOS-HS-BSA. A radioimmunoassay (RIA), using tritiated T-2 toxin and diacetoxyscirpenol (DAS) as marker ligands, was used in screening the hybrid cells. (3) Stability of a cell line capable for the production of monoclonal antibody for the group A trichothecenes was investigated. 4) Efforts to improve the ELISA for diacetoxyscirpenol (DAS) and deoxynivalenol (DON) were made. 5) A new immunoassay involving the use of anti-saxitoxin antibody, biotin-saxitoxin conjugate and avidin-peroxidase complex was developed for saxitoxin. The detection limits of different immunoassays developed in the first year were in the range of 2.5 to 25 pg per assay. Keywords: Phytotoxins, Biological warfare agents. (AW)