Protein-Modified Electrochemically Active Biomaterial Surface

摘要

This research was initiated with a novel approach to electrode immobilization of an enzyme, glucose oxidase, by electropolymerization of pyrrole in the presence of the enzyme. The enzyme was entrapped by the polypyrrole film growing on the electrode surface. Since polypyrrole is an electronically conducting organic polymer, the effect of this immobilization was to enfold the enzyme in a conducting electrode. In addition, the products of the enzyme reaction with glucose were produced in the immediate proximity of this conducting material. The electropolymerized enzyme is active and serves as the basis for a glucose electrode system which remained active for several days. The conditions for electro-polymerization and the characteristics of the immobilized enzyme system were reported in the open literature. (AW)