Cloning and Characterization of the RecA Gene of 'Aquaspirillum magnetotacticum'

摘要

The recA gene of Aquaspirillum magnetotacticum has been isolated from a genomic library and introduced into a recA mutant strain of Escherichia coli K12. The cloned gene complemented both the recombination and DNA repair deficiency of the host and its protein product promoted the proteolytic cleavage of the LexA protein. A protein whose molecular weight is similar to that of the RecA protein of E. coli was associated with the cloned sequence. Keywords: recA gene - Aquaspirillum magnetotacticum - gene library - recombinant cosmid; Gene sequences; Gene mapping. (KT)