Evaluation of Thyroid Uptake (TU) and Total Thyroxine (T4) Tests by Fluorometric Enzyme Immunoassay. (Reannouncement with New Availability Information).

摘要

Fully automated nonradioisotopic assays for the determination of thyroid uptake (TU) and total thyroxine (T4), performed using the Stratus analyzer employing a fluorometric enzyme immunoassay methodology, were evaluated. A microcomputer was interfaced with the analyzer, and a program was written to allow the free thyroxine index (FTI) to be calculated from the TU and T4 values and all results to be printed together, a practical capability which the analyzer lacks. Performance characteristics of the assays were generally acceptable. Patient specimens were analyzed for TU, T4, and FTI by using the Stratus assays (test method) and TDx assays (reference method), which use a fluorescence polarization immunoassay methodology. In addition to the typical statistical means of analyzing method comparison data (scatterplot, linear regression analysis, correlation coefficient), an alternative method of assessing agreement between methods, difference analysis, was used.