BOAA Selectively Enhances L-Glutamate Release from Guinea Pig Hippocampal Mossy Fiber Synaptosomes.

摘要

The neurotoxic amino acids Beta-N-oxalylamino-L-alanine (BOAA) and Beta-N-methylamino-L-alanine (BMAA) were evaluated for possible effects on spontaneous and stimulus-evoked release of L-glutamate (L-Glu) and dynorphin A(1-8)-like immunoreactivity (LI) from guinea pig hippocampal mossy fiber synaptosomes. BOAA (200 microns), but not BMAA (1 mM), was found to significantly increase both basal cytosolic and KC1-stimulated vesicular release of L-Glu. Neither BOAA nor BMAA had any effect on dynorphin A(1--8)-LI release from these synaptosomes. This is the first report describing a presynaptic facilitatory action of BOAA upon L-GLu release; an effect which may contribute to the neurotoxic properties of this proposed environmental toxin. Reprints. (JES)