Anapylactoid Reactions Mediated by Autoantibodies to Cholesterol in Miniature Pigs.

摘要

Autoantibodies to cholesterol were detected and purified from normal (nonimmunized) pig serum. The antibodies were assayed by enzyme-linked immunosorbent assay with crystalline cholesterol as an antigen and by complement-dependent damage to cholesterol-laden liposomes. Intravenous injection of liposomes containing cholesterol into anesthetized animals caused decreased hemolytic complement titers, and induced a reaction consisting of transient neutropenia, thrombocytopenia, respiratory distress, cyanosis, pulmonary and systemic hypertension, and decreased cardiac output. Plasma levels of thromboxane B2 and 6-keto-prostaglandin F1a increased 1300% and 200%, respectively, and leukocyte and platelet counts decreased by 36% and 38% respectively. Injection of cholesterol-free liposomes did not induce the reaction. (jes)