Long Stress-Responsive Non-Coding Transcript (NiT 5) and Its Role in the Development of Breast Cancer.

摘要

The goal of this idea award was to characterize one novel long non- coding transcript that we had identified in a screen using whole-genome tiling arrays. This transcript which is called Long Stress-Induced Non-Coding Transcript 5 (LSINCT5) was one of 12 long non-coding transcripts that we had identified. This transcript was chosen over the other 11, as it had increased expression in most of the breast cancer cell lines and primary tumors that we analyzed. There were three Specific Aims to our original proposal aimed at characterizing this transcript and determining what role it played in the development of breast cancer. While we have been successful in completing most of our original Specific Aims we have struggled in the past year because the level of expression of this transcript is so low. This has made the characterization of this transcript difficult, but we have now been able to reproducibly measure this transcript. In the final year of this Idea Award we propose to finish up the remaining work from our original Specific Aims, but also have several additional things we d like to do in order that we can successfully compete for extramural funding from the National Institutes of Health.