Fourteen-Day Subacute Intravenous Toxicity Study of Hypertonic Saline/Dextran 70 and Its Constituents in Beagle Dogs.

摘要

The subacute toxicity following intravenous administration of a proposed resuscitation fluid, hypertonic saline/Dextran 70 (HSD), was evaluated in male and female beagle dogs. Animals received intravenous doses of HSD, at levels of 12, 16, and 20 ml/kg/day over a 5-minute period, daily for 14 days. Equal volumes of each HSD component, 7.5% hypertonic saline (HS) and 6% Dextran 70 (D70) in normal saline, were also evaluated. Ringer's lactate (RL), dosed at 20 ml/kg/day, served as the control. Blood samples were collected for serum chemistry and hematologic analyses on Day 0 (baseline), Days 1, 2, 3, and 7 before daily administration of the dosing solutions, and Day 14 before necropsy. Clinical signs were observed with increased frequency in the HSD- and HS-treated groups and included disorientation, inactivity, tremors, vomiting, excessive thirst, hunched posture, increased salivation, increased respiratory depth or rate, and panting. The D70-treated animals exhibited signs with incidence intermediate to HSD- or HS-treated animals, and those treated with RL. Since the proposed therapeutic dose of HSD is a single dose of only 4 ml/kg, these findings indicate minimal adverse effects should be anticipated with the therapeutic administration of HSD. Keywords: Subacute toxicity; Intravenous administration; Hypertonic Saline; Resuscitation fluid; Dogs. (edc)