Fibrinogen Recovery in Two Methods of Cryoprecipitate Preparation.

摘要

The purpose of this study was to determine if a relationship exists between the loss of fibrinogen and factor VIII and the volume of supernatant plasma removed during the preparation of cryoprecipitate from fresh frozen plasma. This was accomplished by measuring the fibrinogen and factor VIII content of aliquots of supernatant plasma collected at set points as it was removed. The aliquot collection points were percentages of the total volume of each unit. The amount of fibrinogen and factor VIII was then compared to the total amount of the constituents in the starting plasma and final cryoprecipitate. Results indicated a significant difference between the methods in fibrinogen yield, factor VIII yield and final volume; the slow thaw method being superior to the quick thaw. An inverse linear relationship existed between fibrinogen loss and supernatant plasma volume in the quick thaw method, though the relationship is too small to be of practical value. No linear relationship was established between supernatant volume and the fibrinogen loss in the slow thaw method or factor VIII in either method. The model was able account for all of the fibrinogen and factor VIII in the original starting plasma in the quick thaw method, but only 67% of the fibrinogen and 745 of the factor VIII in the slow thaw method. (SDW)