Human Erythrocytes Have Binding Sites for Beta-Endorphin.

摘要

Monoiodinated human beta-endorphin was found to bind specifically to human erythrocytes. Unlabeled beta-endorphin and beta endorphin inhibited binding, but (-)naloxone, D-Ala, D-Leu-enkephalin, and leu-and metenkephalin did not. Immunoelectron microscopy, using rabbit anti-endorphin antibody, an anti-rabbit lgG secondary antibody, and complexed horseradish peroxidase, revealed that at low concentrations beta-endorphin binds to the cell surface. Electron spin resonance spectroscopy showed no effect of beta-endorphin on membrane fluidity. This receptor does not appear to conform to the characteristics of an opiate receptor. Reprints. (JS)