Evaluation of the Potential of Inhaled Chloropentafluorobenzene to Induce Toxicity in F-344 Rats and B6C3F1 Mice and Sister Chromatid Exchanges and Micronuclei Formation in B6C3F1 Mice.

摘要

Chloropentafluorobenzene (CPFB) has been selected as a candidate material for use as a CW simulant for training purposes. Preliminary screening has indicated that CPFB provides good detectability for biological monitoring, desirable partitioning in biological tissues, acceptable physical properties, and relative biological inertness. Ten Fischer 344 rats and six B6C3F1 mice of each sex were exposed to air, 0.25, 0.80, or 2.50 mg chloropentafluorobenzene (CPFB)/L of air for 3 weeks, excluding weekends. Exposure to 2.50 mg/L caused a reduction in the growth rate of rats but did not affect the growth rate of mice. Following the exposure there was reduced serum glutamic oxaloacetic transaminase activity in the blood serum of exposed rats and a dose related increase in liver weights. Increased liver weights were observed in mice as well; the response in the female groups was clearly dose dependent. Histologically the livers of both rats and mice presented single cell necrosis. In exposed mice hepatocytes exhibited mild hepatocytomegaly with increased granular eosinophilic cytoplasm. In evaluations for its potential to induce chromosomal damage following this exposure regime, CPFB did not alter the rate of bone marrow cellular proliferation. Assessment of the micronucleated polychromatic erythrocytes and normochromatic erythrocyte populations during the inhalation exposures indicated a general absence of genotoxic activity.