Interactions of Plasmodium berghei Sporozoites and Murine Kupffer Cells in Vitro. (Reannouncement with New Availability Information).

摘要

Malaria sporozoites must leave the bloodstream and cross a layer of sinusoidal lining cells in order to infect hepatocytes and undergo exoerythrocytic schizogony. To determine whether Kupffer cells (KC) derived from this layer interact with sporozoites, murine KC were isolated from perfused livers of BALBcJ mice and incubated in vitro with Plasmodium berghei sporozoites. Isolated KC had characteristic macrophage surface Ag and were phagocytic, ingesting both latex particles and Leishmania major amastigotes. In the absence of immune serum, sporozoites associated with fewer than 10 % of these KC. By 30 min, 10 % of the cell-associated sporozoites were completely ingested, 30 % were in the process of being ingested, and 60 % were attached to the surface of the cells.