Structure of Patch-Clamped Membranes in High Voltage Electron Microscopy.

摘要

The study of single ion channel kinetics in cell electrophysiology has been made possible by the introduction of patch-clamp techniques. Recordings can be made from a patch of membrane either attached to the cell or existed into controlled solutions. Though this technique has been widely used for more than a decade, the structure of the membrane patch and its associated cytoplasmic elements are not known except for recent work done in this laboratory. We have improved this technique to visualize membrane patches using high voltage electron microscope and also identified one class of channels in the patch by immunocytochemistry. Reprints. (kt)