Studies of Wound Healing in the Presence of an Angiogenesis Factor. Final Report June 1, 1987-May 30, 1989.

摘要

Basic fibroblast growth factor (bFGF) was found to increase wound healing in a healing-impaired model; the db/db mouse. When bFGF was applied to 6 mm punch biopsy wounds, a dramatic increase in granulation tissue formation was observed. bFGF-treated wounds appeared very similar to control normal wounds in respect to dermal thickness, compactness of dermis, numbers of migratory cells and number of capillaries. No effect on the epidermis was observed. We conclude, therefore, that bFGF may be a useful modality for the treatment of wounds in healing-impaired or healing-compromised patients. Streptozotocin treated mice showed no increase in the rate of healing when treated with bFGF. Diabetic mice (db/db) did show a decrease in rate of wound healing when compared to heterozygous controls. These mice had an increase in the dermal parameters of wound healing when exposed to bFGF. The effect was specific, dose dependent and was eliminated by boiling the bFGF or incubation with antibodies to bFGF. Keywords: Angiogenesis growth factors. (aw)