Effect of D-Mannose on Thymocyte Proliferation Induced by Interleukin-1.

摘要

The effects of various carbohydrates of the response of murine T-cells to cytokines were examined. Concentrations of 4-10mM of the monosacchraide, D-mannose, significantly inhibit thymocyte proliferation induced by either recombinant interleukin-1 (rIL-1) or the native, glycosylated IL-1. Inhibition is specific for D-mannose and methyl-alpha-D-mannopyranoside. Other carbohydrates, including L-mannose and D-glucose exhibit no inhibitory effect on thymocyte growth. Thymocyte proliferation, induced by either IL-2 or pokeweed mitogen, is also inhibited by D-mannose. D-mannose is effective in inhibiting T-cell proliferation, even when added 48 hours after the start of the experiment. D-mannose did not appear to be toxic to the thymocytes as judged by trypan blue exclusion. These data are consistent with the hypothesis that specific carbohydrates may alter immune function. Manipulation of immune function via blocking cytokine activity may be useful in certain pathological conditions, as septic shock. Keywords: T lymphocytes; Immunochemistry; Reprints. (AW)