Plasma Clearance and Tissue Distribution of Dextran Following Infusion of 7.5% NaCl/6% Dextran-70 Euvolemic and Hemorrhaged Rabbits.

摘要

Dextran metabolism was evaluated in euvolemic and hemorrhaged rabbits following administration of a 7.5% NaCl/6% Dextran-70 (HSD) solution. Control rabbits and those bled 8 ml/kg body weight were infused i.v. with 4 ml/kg of HSD or HSD containing c-Dextran-70. Blood samples were withdrawn prior to and 0.17, 0.5, 1, 2, 4, 6, 24, 48, 72, and 96 hours after HSD infusion. Although peak serum dextran concentrations were about 29% higher in hemorrhaged rabbits than in controls, in both groups of rabbits dextran was cleared from serum with a half-life of about 7.4 h. In addition, dextran was distributed throughout the blood volume and did not bind to serum proteins. At the end of the 96 h experimental period, concentrations of radiolabeled dextran were 20-fold higher in liver from both groups of rabbits, in comparison to spleen, lung and kidney. The c-dextran in liver was associated with the cytosolic fraction and was not associated with any membrane structures. Molecular weight distribution of the serum dextran revealed no significant metabolism over the 6 h post-infusion period assayed. After 96 h, radiolabeled dextran in liver showed some degree of metabolism and dextranase activity in liver was markedly higher than in the other tissues assayed. (sdw)