Effects of Hypertonic Saline (7.5%) Dextran-70 (HSD) on Human Red Cell Typing, Lysis, and Metabolism in Vitro.

摘要

The introduction of a 7.5% hypertonic saline/6% Dextran- 70 (HSD) solution into clinical trials for the treatment of hypovolemic states, and the past concerns regarding possible interference of dextran with blood serology, prompted us to investigate the effects of HSD on human red cell typing and stability. HSD was evaluated with fresh and 35-day stored CPDA-1 red cells from 12 healthy donors. A 1:5 mixture of HDS to blood had no effect on ABO, Rh, and MN typing in both fresh and stored blood. HSD produced no significant lysis with fresh cells and a minimal level with stored blood. No evidence of metabolic or morphologic changes were seen after HSD treatment. The results of this study suggest that clinical use of HSD for treatment of hemorrhagic shock will not affect blood group determinations or red cell stability from stored blood which may be infused after the HSD treated patient is transported to a hospital. Keywords: Hypertonic saline; Dextran-70; Red cell typing; Red cell lysis; Blood storage. (kt)