Reversible and Irreversible Blue Light Damage to the Isolated, Mammalian Pigment Epithelium. (Reannouncement with New Availability Information).

摘要

Most studies have employed cell damage or death as a means of evaluating the photo-toxicity of blue light on the pigment epithelium. We chose to investigate the effects of blue light on the functional properties of the isolated bovine pigment epithelium as manifested in the electrical parameters and transport of leucine and chloride. Figure 1. The picture of the Using chamber shows where the tissue is placed (a), the position of the voltage (b) and current electrodes (c) as well as the thermostatically controlled heating elements (d) inserted into the sides of the chamber. The pigment epithelium (PE) with tapetal and choroidal remnants was isolated from perfused cow eyes and mounted in a chip as a membrane separating two compartments of a Using Chamber. The voltage sensing electrodes are in close opposition to the tissue whereas the current electrodes are located in the distal ends of the chambers. The thermostatically controlled heating elements maintain the bicarbonate buffered saline solution at 34 C.