Role of the Wasp Venom Peptide Mastoparan in the Study of Mechanisms Involved in Cell Death.

摘要

The effect of mastoparan on membrane function in Madin Darby Canine Kidney (MOCK) cells was studied. Mastoparan increased [ 3 H]- inositol phosphates formation 56% (p<.001), 172% (p<.001), and 231% (p<.001} following treatment of 25, 75, and 100 ugjml mastoparan, respectively. Mastoparan-induced LDH release reached 8% at 1 min (p<.005) and 32% at 15 min (p<.001). Mastoparan also mediated the accumulation of inositol-trisphosphate with a concommitant decrease in the level of phosphatidylinositol-bisphosphate. Furthermore, mastoparan significantly activated (p<.001) phosphatidylinositol and phosphatidylinositol-phosphate kinase activities in a time and dose dependent fashion. These data suggest that the cellular toxic effects of mastoparan may in part be due to changes in phospholipid metabolism, thereby altering membrane structure and function.