(Staphylococcal Aureus Enterotoxin A): Partial Characterization of an N-Terminal Peptide

摘要

In the present work, we report the production of specific anti-SEA sera using an N-terminal synthetic peptide derived from the SEA molecule (SEAp). The rationale for selecting this part is that molecular termini are conformationally less restricted in the native protein and, therefore, might be recognized by a larger population of antibodies to the peptide. A peptide from the N-terminal sequence of staphylococcal enterotoxin A (SEA) was synthesized and conjugated to bovine serum albumin (BSA). When injected into rabbits, this conjugate induced antibodies which reacted with the native SEA molecule. These antibodies did not cross-react with other enterotoxins. However, the obtained anti-peptide antibodies were less efficient in detecting SEA than antibodies raised to the whole molecule. (kt)