Evaluation of Enhanced Luminescence Immunoenzymometric Assays (LIA) for Ferritin and Free T4

摘要

The Amerlite enhanced luminescence immunoenzymometric as says (LIA) for ferritin and free T4 (FT4) have been evaluated. The LIA assay system employs a two-site binding approach in which one antibody is coated on the interior of plastic microtiter wells and another antibody, or antigen, is tagged with horseradish peroxidase (HRPO). Signal, proportional to the amount or patient analyte, is produced when the HRPO catalyzes the oxidation of luminol resulting in light production. The light signal is increased in magnitude by the incorporation of an enhancing agent which also greatly prolongs the lifetime of the signal. Light production is monitored by an automated luminometer which uses a stored calibration curve to calculate patient results. Keywords: Reprints, Thyroid status, Iron deficiency anemia, Nonradioisotopic immunoassay; Luminescent immunoassay.