Alterations in Splenic and Hepatic Protein Kinase C in Sepsis and Chronic Endotoxemia

摘要

Tissue resistance to various hormones, in particular catecholamines, is a fundamental problem in sepsis, that often prevents the successful resuscitation of septic patients. In order to devise more effective therapy, it is essential to determine the pathophysiology of this tissue resistance. It appears that sepsis and chronic endotoxemia specifically alter signal transduction system coupled to PKC, resulting in a down regulation of PKC-linked receptors and an attenuation of phosphoinositide turnover. This study was therefore designed to determine the effects of sepsis and chronic endotoxemia on hepatic and splenic PKC. In summary, this study has defined a relatively simple method of delineating phorbol ester receptors within the spleen and liver. Further, it was shown that sepsis and chronic endotoxemia markedly. Reprints. (kt)