Advanced Biosensors for Amino Acid Detection.

摘要

Experimental work performed during this program demonstrated that amino acids (phenylalanine and 3-hydroxytyrosine) could be reversibly, quantitatively and selectively detected on evanescent fiber optic sensors using immobilized acyclic polyether hosts supported on their outside surface. This investigation suggested that optical detection of the subject molecular recognition event may occur in the absence of a distinct absorbance change and be a probable consequence of changes in refractive index or dielectric properties at the interfacial region. This implied that the optical molecular recognition associated with interaction between the evanescent wave and host/guest chemistry at a fiber optic waveguide can be extended to include the probing of more subtle optical changes at the fiber optic waveguide/host interfacial region. (jes)