Enzyme-Linked Immunosorbent Assays for Kinins Using High-Affinity Monoclonal Kinin Antibodies.

摘要

The kallikrein-kinin system has been implicated as having a role in a number of physiological and pathological conditions. Antagonists have been developed for the known types of kinin receptors, B1 and B2. However, these antagonists are peptides with relatively low affinities for kinin receptors and may have a short duration of action in vivo due to their inactivation by peptidases and/or their removal from the circulation by renal excretion. The purpose of the present study was to prepare antibodies reactive with kinins that could be used in experiments to block the actions of kinins by competing with kinin receptors for the available kinin or that could be used in experiments to elicit anti-idiotypic antibodies that block kinin receptors by cross-reacting with the kinin binding sites of these receptors. Hybridoma technology was applied in the production of kinin antibodies so that an unlimited supply of antibodies would be available for future experiments. This paper describes the production and characterization of monoclonal antibodies reactive with kinins. (js)