Detection and Identification of Vipera Russelli Venom.

摘要

The highly sensitive fluorogenic Enzyme-linked Immunosorbent Assay (FELISA) has been adapted for the detection and identification of Vipera russelli venom. The assay sensitivity was observed to be 10 to the minus 13th power g ml to the (-1) power. Venoms from snakes of the Vipera group exhibited a high degree of cross reactivity when tested with the anti-body raised against V. russelli venom. With the exception of venom from Naja naja, all the tested venoms from unrelated families also showed cross reactivity. This procedure is useful for detecting snake venom or its components in biological samples. Keywords: Snake venom, Fluorogenic ELISA (FELISA), Nitrocellulose membrane, Cross reactivity, Immunoassays. (kr)