Genotoxicity Assessment of Mixed Oligomers of Chlorotrifluoroethylene Using a Battery of In vitro and In vivo/In vitro Assays.

摘要

Halocarbon 3.1 oil, a potential hydraulic fluid consisting of mixed CTFE oligomers, was evaluated in vitro boassays to assess its potential genotoxic activity. The assays conducted were the Salmonella/Reverse Mutation Assay, the Chinese Hamster Ovary/Forward Mutation Assay, Sister Chromatid Exchange and Chromosome Aberration Assay, the BALB/c-3T3 Cell Transformation Assay an In Vivo/In Vitro Unscheduled DNA Synthesis Assay and S-Phase Synthesis Assay. CTFE oligomers caused a weak evaluation in the mutant frequency in the forward mutation assay without metabolic activation and an increase in S-phase DNA synthesis in rat liver. The evaluation in the mutant frequency was considered too tenuous to warrant any consideration in risk assessment. Because CTFE was negative in all other assays, the results of this test battery would predict no genetic risk from CTFE however, the results from the S-phase synthesis assay indicate that the test article is hepatotoxic.