Detection of Microbial Trypsin-Like Enzymes by Use of an Agar Gel.

摘要

Certain oral micro-organisms associated with periodontal diseases elaborate trypsin-like enzymes. This study describes a simple assay for these enzymes, based on the incorporation of the synthetic substrate N-Alpha-benzoyl-DL-arginine-p-nitroanilide (BAPNA) into ion-agar gel. The system can provide quantitative or qualitative data within 24 h and is suitable for use in the dental clinic.