Modeling of the Non-Auditory Response to Blast Overpressure. Autologous PerfusionTechnique for an Isolated Gastrointestinal Tract of a Rabbit

摘要

Most perfusion techniques rely on mechanical means to provide blood flow to theisolated organ for maintaining its physiological conditions. The approach usually requires a complicated mechanical system with the associated problems of blood type matching and prevention of blood cell damage. This paper describes a gastrointestinal tract perfusion technique developed to use the rabbit's own cardiopulmonary system as the autologous blood supply source. The technique involves the removal of the complete intestinal loop, duodenum to descending colon, from the abdominal cavity of the rabbit, and maintaining its blood circulation through silastic tubing connections of the catheterized portal vein and cranial and caudal mesenteric arteries. The isolated perfused GI tract may then be placed in a separate test environment for controlled experiments. For a terminal animal test, the approach was found to be a convenient alternative to the conventional approaches. (jes)