Measurement of Intracellular Ionized Calcium and Membrane Potential

摘要

Measurement of intracellular ionized calcium concentration (Ca2+)i) in livingcells is of considerable interest to investigators over a broad range of cell biology. Calcium has an important role as a mediator of transduction of signals from the cell membrane; elevations in (Ca2+)i are part of the regulation of diverse cellular processes. Eukaryotic cell have an internal calcium ion concentration that is usually maintained at 100 nM, far below the extracellular environment, by regulation of calcium channels within the plasma membrane and storage and release of Ca2+ from sites such as calciosomes, endoplasmic reticulum and mitochondria. (jes)