Flow Cytometric Ploidy Determination of Oral Premalignant and Malignant Lesions

摘要

Nuclear DNA content was evaluated for use as an objective parameter of diagnosticvalue in oral premalignancy and malignancy. Fifty-three blocks of formalin-fixed and paraffin-embedded archival tissue were selected from 20 cases which has been diagnosed as premalignant epithelial lesions and subsequently diagnosed as having progressed to malignancy. A single cell suspension was prepared from each tissue block, stained with propidium iodide and subjected to flow cytometric analysis. This yielded histograms which depicted the ploidy status for each specimen. For five specimens, the tissue quantity was insufficient and for an additional six specimens, the coefficient of variation for the histogram exceeded the established limit of seven. (JES)