Development and Evaluation of Adeno HTLV-III Hybrid Virus and Non-Cytopathic HTLV-III Mutant for Vaccine Use

摘要

Conditions were established and standardized for reproducibly infectingCercopithecus with HIV-2 in the laboratory. Cercopithecus infected and vaccinated with HIV were monitored for an immune response on the basis of antigen-induced cellular proliferation and development of an antibody response detectable by Western blot assay. The anti-HIV antibody was also characterized for its ability to neutralize HIV virus. Wild Cercopithecus were tested for naturally occurring HIV infection and retroviruses isolated from five of these animals have been propagated in vitro. After screening and two cycles of plaque purification, four recombinant HIV gp120/adenovirus 2 isolates were obtained that produced HIV envelope protein as determined by immunocytochemical staining using a rabbit antibody directed against gp120. The HIV gp120 sequences in one of these were incorporated into the early region 1B rather than into the targeted early region 3. Keywords: AIDS; Vaccine; Recombinant DNA; Antibody; T-cell. (jes)