Binding Assays for the Quantitative Detection of P. Brevis Polyether Neurotoxinsin Biological Samples and Antibodies as Therapeutic Aids for Polyether Marine Intoxication

摘要

The polyether lipid-soluble toxins isolated from the marine dinoflagellatePtychodiscus brevis can be detected using a number of different types of immunosorbant assay. A comparison of six different formats were undertaken, evaluating each of sensitivity and for background color development. Further purification of specific anti-brevetoxin antibody, followed by utilization in four different types of immunosorbant assay, indicated that a sandwich-type assay employing toxin adsorbed to plates, specific antibrevetoxin antibody, and antispecies-antibody covalently linked to peroxidase enzyme as primary, secondary, and tertiary adsorbant could yield sensitivities in the 40 pgram to 40 ngram concentration ranges. The stabilities of each of the reagents used in the latter 'sandwich' assay were evaluated and checkerboard protocols were undertaken to optimize the assay. There is no cross-reactivity from the okadaic acid type polyether toxins, but substantial cross-reactivity with ciguatoxin. Final results will be summarized in the final report. Keywords: Sodium channels, Receptor binding. (KT)