Antineoplastic Agents, 177. Isolation and Structure of Phyllanthostatin 6

摘要

The isolation and structural elucidation of a new phyllanthus glycoside,phyllanthostatin 6, was summarized. Phyllanthostatin 6 was isolated from the roots of Phyllanthus acuminatus (Euphorbiaceae) and was found to inhibit (ED50=.35 ug/ml) growth of the murine P-388 lymphocytic leukemia cell line. Two other new constituents were shown to be didesacetylphyllanthostatin 3 and descinnamoylphyllanthocindiol. Structure determinations were achieved employing hrfabms and 2D-nmr spectroscopy. Application of an HPLC separation technique to the Phyllanthus glycosides and development of a new isolation procedure for the major antineoplastic constituent, phyllanthoside, are also described.