Flow Cytometric Measurement of Intracellular Ionized Calcium in Single Cells withIndo-1 and Fluo-3

摘要

Measurement of intracellular ionized calcium concentration (Ca2+i) in livingcells is of considerable interest to investigators over a broad range of cell biology. Calcium has an important role in a number of cellular functions and, perhaps most interestingly, can function to transmit information from cell membrane to regulate diverse cellular functions. An optimal indicator should span the range of calcium concentrations found in quiescent cells to levels measured in stimulated cells with greatest sensitivity to small changes at the lower end of range. The indicator should freely diffuse throughout the cytoplasm and be easily loaded into small cells.