Assessment of 7.5% NaCl /6% Dextran-70 (HSD) Effects on Serum or Plasma ProteinDeterminations

摘要

For the past 25 years, reports have appeared in the literature citing dextraninterference with accurate plasma or serum protein determinations, particularly as assayed by the biuret method. In our evaluations of the overall effects of HSD, we have monitored changes in serum or plasma protein concentrations following hemorrhage and subsequent resuscitation. The present study investigates protein concentrations in human, rabbit, and swine serum or plasma following a single i.v. infusion of 7.5% NaCl/6% Dextran-70 (HSD) at 4 ml.kg, or daily doses at the maximum tolerated dose of 16 ml/kg for 14 days. Protein concentrations were determined by modified Lowry, dye-binding, and an automated biuret method, as well as by refractometry, before, and at various times following HSD infusion in both euvolemic and hemorrhaged animals. Other studies analyzed plasma protein concentrations from 13 human trauma patients infused with 250 ml of HSD.