Application of Chemiluminescence for the Detection of Thiodiglycol in AqueousMatrices Using High-Performance Liquid Chromatography

摘要

A new high-performance liquid chromatography (HPLC) method is described toanalyze trace amounts of thiodiglycol (TDG) in surface water, seawater, soil extracts, and crushed concrete extracts using fluorine induced sulfur chemiluminescence detection (SCD). Sulfur chemiluminescence detection is a new detection technique that is highly selective for reduced organosulfur compounds. Separation of TDG from the aqueous matrix is carried out on a RP-18 narrow bore (220 x 2.1 mm i.d.) column using an isocratic mobile phase consisting of 3% acetonitrile and 97% water. Detection response was found to be linear over a concentration range of three orders of magnitude (4-400 ng injected) with the minimum detection limit being approximately 4 ng (S/N = 3). Keywords: Chemiluminescence detection, Seawater analysis, Environmental analysis, Mustard