Isolation of a New Herpesvirus from Human CD4+ T Cells.

摘要

A new human herpesvirus has been isolated from CD4(+) T cells purified from the peripheral blood mononuclear cells of a healthy individual (RK), following incubation of the cells under conditions promoting T-cell activation. The virus could not be recovered from nonactivated cells. Cultures of lymphocytes infected with the RK virus exhibited a cytopathic effect, and electron microscopic analyses revealed a characteristic herpesvirus structure. RK virus deoxyribonucleic acid did not hybridize with large probes derived from herpes simplex virus, Epstein-Barr virus, varicella-zoster virus, and human cytomegalovirus. The genetic relatedness of the RK virus to the recently identified T-lymphotropic human herpesvirus 6 (HHV-6) was investigated by restriction enzyme analyses using 11 probes derived from two strains of HHV-6 (Z29 and U1102). Keywords: Human herpesvirus, CD4+ Human T cells, Latency, T cell activation, Virus latency, Reprints, Microbiology. (jg)