Cultivation of Sexually Mature Brugia malayi in vitro.

摘要

Sexually mature male and female Brugia malayi were development from third-stage larvae after 60 days in the in vitro culture system described by Franke and others in 1987 (Am J Trop med Hyg 37:370-375). Between 75 and 100 days in culture, many worms produced living microfilariae. Each gravid female produced 200=1,500 microfilariae/day. Keywords: Brugia malayi, Biology, Wuchereria bancrofti, In vitro culture. (JS)