Physiologically Relevant Pharmacokinetic Model of Xenobiotic PercutaneousAbsorption Utilizing the Isolated Perfused Porcine Skin Flap

摘要

The goal of modeling percutaneous absorption using in vitro systems is toquantitatively predict the disposition of topically applied chemicals in vivo and to gain an insight into the physiological mechanisms involved in the transit of the compound through skin. To optimally achieve both of these goals, the in vitro system utilized should be as anatomically and physiologically similar to in vivo skin as possible and should generate data which are amenable to quantitative analysis. The isolated perfused porcine skin flap (IPPSF) is an anatomically intact alternative animal model for skin penetration studies and possesses a viable epidermis and an accessible and functional microcirculation. Previous studies have demonstrated the utility of the IPPSF to investigate percutaneous absorption of xenobiotics, and the ability to manipulate the cutaneous microcirculation by the transdermal administration of vasoactive drugs.