Interaction of Leukotriene C4 and Chinese Hamster Lung Fibroblasts (V79A03Cells). 2. Subcellular Distribution of Binding and Unlikely Role of Glutathione-S-Transferase

摘要

It was reported previously that radiation-induced cytotoxicity in V79A03 (V79)cells was attenuated by pretreatment of cells with leukotriene C4 (LTC4), leading us to determine that V79 cells possessed specific binding sites, with characteristics of receptors, for LTC4 (see the preceding companion communication). Additional studies were conducted to determine the subcellular distribution and the chemical nature of the LTC4 binding assays resulted in a 74% reduction in high-affinity binding, plasma membrane and nuclear fractions were obtained from V79 cells.