Genetically-Engineered Poxviruses and the Construction of Live RecombinantVaccines

摘要

A survey of the transcript levels associated with vaccinia virus was performed.Specific and unique vaccinia promoters were identified by standard techniques and manipulated to drive the expression of foreign genes such as Beta lactosidase and glycoproteins. Promoters of all three classes (early, intermediate, late) were identified and studied. No single promoter was found to be exceptional. Most strong promoters C10LW, H6, 11K, IL3 gave good expression which fell within several fold of each other. No promoter was found that gave magnitude increased levels of expression. Generally, irrelevant proteins such as Beta galactosidase could be expressed at high levels sufficient to visualize on Coomasie stained gels. Unfortunately, this was not the case when relevant proteins such as viral glycoprotein were expressed. The additional factors that need to be involved for high level expression by vaccinia virus vectors are still unknown.(TTL)