Development of an Aquatic Bioassay for Carcinogenicity and Toxicity Testing Usingthe Medaka (Oryzias latipes) as a Model

摘要

The primary objective of this study was to help to establish the aquatic bioassayas a valid alternative to rodent carcinogenicity and toxicity testing for use by the U.S. Army to test contaminated ground water and effluents in a rapid, inexpensive manner. The study had two parts: the first to study normal hepatic parenchymal development in the fish species to be used, the medaka, and the second to perform a controlled carcinogenic exposure study using the medaka with documentation of the lesions and neoplasms produced. The results outlined in this report have shown that the protocol used is potentially a rapid method of testing carcinogens using the medaka as a model. Keywords: Medaka; Oryzias latipes; Diethylnitrosamine; Neoplasm, Carcinogen; Liver; Hepatocyte; Ultrastructure. (js)