Assay of Follitropin and Lutropin by Fluorescence Enzyme Immunoassay

摘要

Recently developed fluorescent enzyme immunoassay (FEI) for follitropin (FSH) andLutropin (LH) designed for the Stratus analyzer were compared to a typical dual FSH/LH RIA procedure. The FEI assays use monoclonal antibodies in a sandwich reaction scheme similar to that of immunoradiometric procedures, but using filter paper tabs rather than polystyrene beads and alkaline phosphatase as a tag instead of a radioisotope. Precision of the FEI assays was comparable to that of the RIA tests with CVs typically under 10%. The detection limit of the FSH test is 0.4 IU/L and that of the LH test is 0.6 IU/L. Measurable carryover exists, but is clinically inconsequential. Neither assay exhibits total specificity but the degree of crossreactivity is unlikely to be problematic in most situations. Recovery with the FSH assay was 103%, and with the LH assay averaged 108%. Moderate amounts of bilirubin and triglycerides and gross hemolysis caused no significant interferences. Method comparison studies yielded the following: FEI