'Coxiella Burnetii' Vaccine Development: Lipopolysaccharide Structural Analysis.

摘要

Analytical instrumentation and methodology are presented for the determination of endotoxin-related structures at much improved sensitivity and specificity using techniques in chromatography and mass spectrometry. These techniques include a newly synthesized reagent that has provided femtomole sensitivity for oligosaccharide detection; and a preanalysis chemical approach for the determination of oligosaccharide linkage, branching, and sequence. These approaches are compatible with supercritical fluid chromatography-mass spectrometry (SFC-MS) and combine to provide the elements of a global approach to oligosaccharide structure. The utility of this approach has been demonstrated for glycolipids and N-linked glycans. The lipid-A structure from Salmonella minnesota has been demonstrated and the report outlines the currently understood structure Coxiella burnetii lipid-A.